Membrane characterization of amastigote-like forms ofLeishmania donovani
نویسندگان
چکیده
منابع مشابه
Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms.
A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigote-like forms in Schneider's Drosophila medium supplemented with 20% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession ...
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We report the cloning and molecular analysis of the Leishmania donovani clpB gene. The protein-coding region is highly conserved compared with its L. major homologue, while 5'- and 3'-flanking DNA sequences display considerable divergence. The encoded mRNA has an unusually long 5'-leader sequence typical for RNAs, which are translated preferentially under heat stress. The gene product, a 100-kD...
متن کاملDetection of amastigote-like forms in the valve of Phlebotomus papatasi infected with Leishmania major.
A massive and homogeneous amount of amastigote-like forms was detected in the stomodeal valve (SV) and the thoracic mid-gut (TMG) of Leishmania major-infected Phlebotomus papatasi, which received a second blood meal 13 to 21 days post-infection on healthy anaesthetized hamsters. After re-feeding, the infected sand flies were dissected out to examine the morphology of the parasite in SV, TMG and...
متن کاملcharacterization of in vitro cultivated amastigote like of leishmania major: a substitution for in vivo studies
background: promastigotes of leishmania spp. have been readily cultured, but the axenic culture of amastigotes has been successful in l. donovani , l .infantum l. mexicana and l. pifanoi . however, some species such as l. major, is much less amenable to axenic cultivation. in present study, we describe an in vitro culture system for the generation and propagation of axenic amastigotes form of l...
متن کاملIdentification of amastigote-specific antigens of Leishmania donovani using kala-azar patient sera.
Antigenic characterization of the soluble fraction of axenic amastigotes of Leishmania donovani ( strain Dd8, causative agent of Indian kala-azar) and their comparison with promastigotes is reported. The axenic amastigotes were assessed for their immunological status employing anti-A2 monoclonal antibody which is extremely specific for L. donovani amastigotes. SDS-PAGE of 35[S] methionine label...
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ژورنال
عنوان ژورنال: Tropical Medicine & International Health
سال: 1996
ISSN: 1360-2276,1365-3156
DOI: 10.1046/j.1365-3156.1996.d01-90.x